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espacepolitique August 16, 2021 Uncategorized 1 Comment Read more

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  • To eliminate cell aggregates, the cell suspension system was passed through a cell strainer (BD Falcon)
  • Transduction of HaCaT cells with lentiviral-based shRNAs targeting (SHVRS-“type”:”entrez-nucleotide”,”attrs”:”text”:”NM_006164″,”term_id”:”1531243743″,”term_text”:”NM_006164″NM_006164) or scrambled non-target bad control (SHC002V) was performed while previously described [36]
  • In contrast, when A549/D16 cells were co-treated with VER and DOC, cytotoxicity was increased significantly on A549/D16 cells, reducing the survival of cells in a dose-dependent manner (Figure 1A)
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  • Subsequently, the original packaging complex inferred from live imaging studies was identified simply by immunoprecipitation of complexes containing Gag and gRNA from cell lysates (Kutluay and Bieniasz, 2010)

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